Precise programming of multigene expression stoichiometry in mammalian cells by a modular and programmable transcriptional system.

ABSTRACT: Context-dependency of mammalian transcriptional elements has hindered the quantitative investigation of multigene expression stoichiometry and its biological functions. Here, we describe a host- and local DNA context-independent transcription system to gradually fine-tune single and multiple gene expression with predictable stoichiometries. The mammalian transcription system is composed of a library of modular and programmable promoters from bacteriophage and its cognate RNA polymerase (RNAP) fused to a capping enzyme. The relative expression of single genes is quantitatively determined by the relative binding affinity of the RNAP to the promoters, while multigene expression stoichiometry is predicted by a simple biochemical model with resource competition. We use these programmable and mo

SUBMITTER: Qin C 

PROVIDER: S-EPMC10023750 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature