High-Throughput Cellular Thermal Shift Assay Using Acoustic Transfer of Protein Lysates.

ABSTRACT: Cellular thermal shift assay (CETSA) is a valuable method to confirm target engagement within a complex cellular environment, by detecting changes in a protein's thermal stability upon ligand binding. The classical CETSA method measures changes in the thermal stability of endogenous proteins using immunoblotting, which is low-throughput and laborious. Reverse-phase protein arrays (RPPAs) have been demonstrated as a detection modality for CETSA; however, the reported procedure requires manual processing steps that limit throughput and preclude screening applications. We developed a high-throughput CETSA using an acoustic RPPA (HT-CETSA-aRPPA) protocol that is compatible with 96- and 384-well microplates from start-to-finish, using low speed centrifugation to remove thermally destabilized pr

SUBMITTER: Owens AE 

PROVIDER: S-EPMC10026039 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature