Fusobacterium nucleatum induces a tumor microenvironment with diminished adaptive immunity against colorectal cancers.

ABSTRACT:

Background & aims

Fusobacterium nucleatum (FN) plays a pivotal role in the development and progression of colorectal cancer by modulating antitumor immune responses. However, the impact of FN on immune regulation in the tumor microenvironment has not been fully elucidated.

Methods

The abundance of FN was measured in 99 stage III CRC tumor tissues using quantitative polymerase chain reaction. Gene expression profiles were assessed and annotated using consensus molecular subtypes (CMS), Gene Ontology (GO) analysis, and deconvolution of individual immune cell types in the context of FN abundance. Immune profiling for tumor infiltrating T cells isolated from human tumor tissues was analyzed using flow cytometry. Ex vivo tumor-infiltrating T cells were stimulated in

SUBMITTER: Kim HS 

PROVIDER: S-EPMC10028079 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature