Presence of immunogenic alternatively spliced insulin gene product in human pancreatic delta cells.

ABSTRACT:

Aims/hypothesis

Transcriptome analyses revealed insulin-gene-derived transcripts in non-beta endocrine islet cells. We studied alternative splicing of human INS mRNA in pancreatic islets.

Methods

Alternative splicing of insulin pre-mRNA was determined by PCR analysis performed on human islet RNA and single-cell RNA-seq analysis. Antisera were generated to detect insulin variants in human pancreatic tissue using immunohistochemistry, electron microscopy and single-cell western blot to confirm the expression of insulin variants. Cytotoxic T lymphocyte (CTL) activation was determined by MIP-1β release.

Results

We identified an alternatively spliced INS product. This variant encodes the complete insulin signal peptide and B chain and an alternative C-terminus that largely

SUBMITTER: van Tienhoven R 

PROVIDER: S-EPMC10036285 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature