Quantify permeability using on-a-chip models in high-throughput applications.
Ontology highlight
ABSTRACT:
SUBMITTER: Soragni C
PROVIDER: S-EPMC10037215 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
Publications
Quantify permeability using on-a-chip models in high-throughput applications.
Soragni Camilla C Vergroesen Tessa T Hettema Nynke N Rabussier Gwenaëlle G Lanz Henriëtte L HL Trietsch Sebastian J SJ de Windt Leon J LJ Ng Chee P CP
STAR protocols 20230120 1
Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%). With on-a-chip barrier models, this assumption fails in cell-free or leaky conditions, which requires the use of the exact solution. To encounter a time delay from performing the assay and acquiring the data, we present a protocol with the exact equation modified to incorporate a t ...[more]
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