Quantify permeability using on-a-chip models in high-throughput applications.

ABSTRACT: Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%). With on-a-chip barrier models, this assumption fails in cell-free or leaky conditions, which requires the use of the exact solution. To encounter a time delay from performing the assay and acquiring the data, we present a protocol with the exact equation modified to incorporate a time offset.

SUBMITTER: Soragni C 

PROVIDER: S-EPMC10037215 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature