Complete sequence verification of plasmid DNA using the Oxford Nanopore Technologies' MinION device.

ABSTRACT:

Background

Sequence verification is essential for plasmids used as critical reagents or therapeutic products. Typically, high-quality plasmid sequence is achieved through capillary-based Sanger sequencing, requiring customized sets of primers for each plasmid. This process can become expensive, particularly for applications where the validated sequence needs to be produced within a regulated and quality-controlled environment for downstream clinical research applications.

Results

Here, we describe a cost-effective and accurate plasmid sequencing and consensus generation procedure using the Oxford Nanopore Technologies' MinION device as an alternative to capillary-based plasmid sequencing options. This procedure can verify the identity of a pure population of plasmid, either

SUBMITTER: Brown SD 

PROVIDER: S-EPMC10039527 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature