Application of a core genome sequence typing (cgMLST) pipeline for surveillance of Clostridioides difficile in China.

ABSTRACT:

Introduction

Clostridioides difficile (C. difficile) is a nosocomial bacterial pathogen that causes antibiotic-associated diarrhea mediated by cellular exotoxins secreted into the intestine during bacterial growth. Multilocus sequence typing (MLST) and PCR ribotyping are the main molecular typing for C. difficile. Whole genome sequencing (WGS) core genome multilocus sequence typing (cgMLST) was developed for genetic evolution and outbreak investigation of C. difficile with higher precision and accuracy.

Methods

A total of 699 whole (complete and draft) genome sequences of distinct C. difficile strains were used in this study to identify core gene set (2469 core genes) and the cgMLST scheme for the phylogeny analysis of C. difficile. This cg

SUBMITTER: Wang YY 

PROVIDER: S-EPMC10040748 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature