MiR-101a-3p Impairs Synaptic Plasticity and Contributes to Synucleinopathy.

ABSTRACT:

Background

Synucleinopathies are disorders characterized by the abnormal accumulation of α-synuclein (aSyn). Synaptic compromise is observed in synucleinopathies parallel to aSyn aggregation and is accompanied by transcript deregulation.

Objective

We sought to identify microRNAs associated with synaptic processes that may contribute to synaptic dysfunction and degeneration in synucleinopathies.

Methods

We performed small RNA-sequencing of midbrain from 6-month-old transgenic mice expressing A30P mutant aSyn, followed by comparative expression analysis. We then used real-time quantitative polymerase chain reaction (qPCR) for validation. Functional analysis was performed in primary neurons by biochemical assays and imaging.

Results

We found several deregulated bi

SUBMITTER: Xylaki M 

PROVIDER: S-EPMC10041420 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature