Carbon dots as a versatile tool to monitor insulin aggregation.

ABSTRACT: The possibility to monitor peptide and protein aggregation is of paramount importance in the so-called conformational diseases, as the understanding of many physiological pathways, as well as pathological processes involved in the development of such diseases, depends very much on the actual possibility to monitor biomolecule oligomeric distribution and aggregation. In this work, we report a novel experimental method to monitor protein aggregation, based on the change of the fluorescent properties of carbon dots upon protein binding. The results obtained in the case of insulin with this newly proposed experimental approach are compared with those obtained with other common experimental techniques normally used for the same purpose (circular dichroism, DLS, PICUP and ThT fluorescence). The

SUBMITTER: Zingale GA 

PROVIDER: S-EPMC10049934 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature