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BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes.

ABSTRACT: Epigenetic modifications of histones are associated with development and pathogenesis of disease. Existing approaches cannot provide insights into long-range interactions and represent the average chromatin state. Here we describe BIND&MODIFY, a method using long-read sequencing for profiling histone modifications and transcription factors on individual DNA fibers. We use recombinant fused protein A-M.EcoGII to tether methyltransferase M.EcoGII to protein binding sites to label neighboring regions by methylation. Aggregated BIND&MODIFY signal matches bulk ChIP-seq and CUT&TAG. BIND&MODIFY can simultaneously measure histone modification status, transcription factor binding, and CpG 5mC methylation at single-molecule resolution and also quantifies correlation between local and distal elements.

SUBMITTER: Weng Z

PROVIDER: S-EPMC10052867 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes.

Weng Zhe Z Ruan Fengying F Chen Weitian W Chen Zhichao Z Xie Yeming Y Luo Meng M Xie Zhe Z Zhang Chen C Wang Juan J Sun Yuxin Y Fang Yitong Y Guo Mei M Tan Chen C Chen Wenfang W Tong Yiqin Y Li Yaning Y Wang Hongqi H Tang Chong C

Genome biology 20230329 1

Epigenetic modifications of histones are associated with development and pathogenesis of disease. Existing approaches cannot provide insights into long-range interactions and represent the average chromatin state. Here we describe BIND&MODIFY, a method using long-read sequencing for profiling histone modifications and transcription factors on individual DNA fibers. We use recombinant fused protein A-M.EcoGII to tether methyltransferase M.EcoGII to protein binding sites to label neighboring regio ...[more]

PMID: 36991510

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