A comprehensive map of hotspots of de novo telomere addition in Saccharomyces cerevisiae.

ABSTRACT: Telomere healing occurs when telomerase, normally restricted to chromosome ends, acts upon a double-strand break to create a new, functional telomere. De novo telomere addition on the centromere-proximal side of a break truncates the chromosome but, by blocking resection, may allow the cell to survive an otherwise lethal event. We previously identified several sequences in the baker’s yeast, Saccharomyces cerevisiae , that act as hotspots of de novo telomere addition (termed Sites of Repair-associated Telomere Addition or SiRTAs), but the distribution and functional relevance of SiRTAs is unclear. Here, we describe a high-throughput sequencing method to measure the frequency and location of telomere addition within sequences of interest. Combining this methodology with a computati

SUBMITTER: Ngo K 

PROVIDER: S-EPMC10055226 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature