Tectonic infarct analysis: A computational tool for automated whole-brain infarct analysis from TTC-stained tissue.

ABSTRACT:

Background

Infarct volume measured from 2,3,5-triphenyltetrazolium chloride (TTC)-stained brain slices is critical to in vivo stroke models. In this study, we developed an interactive, tunable, software that automatically computes whole-brain infarct metrics from serial TTC-stained brain sections.

Methods

Three rat ischemic stroke cohorts were used in this study (Total n = 91 rats; Cohort 1 n = 21, Cohort 2 n = 40, Cohort 3 n = 30). For each, brains were serially-sliced, stained with TTC and scanned on both anterior and posterior sides. Ground truth annotation and infarct morphometric analysis (e.g., brain-V_brain, infarct-V_infarct, and non-infarct-V_non-infarct volumes) were completed by domain experts. We us

SUBMITTER: Santo BA 

PROVIDER: S-EPMC10070917 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature