Ontology highlight
ABSTRACT: Significance
Currently, the translation of apolipoprotein biomarkers to clinical application is impaired because of the high cost of large cohort studies using traditional single-analyte immunoassays. The application of on-line tryptic digestion coupled with LC-MS/MS analysis is an effective way to address this problem. In this work we demonstrate a high throughput, multiplexed, automated proteomics workflow for the simultaneous analysis of multiple proteins.
SUBMITTER: Toth CA
PROVIDER: S-EPMC10071838 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Toth Christopher A CA Kuklenyik Zsuzsanna Z Jones Jeffrey I JI Parks Bryan A BA Gardner Michael S MS Schieltz David M DM Rees Jon C JC Andrews Michael L ML McWilliams Lisa G LG Pirkle James L JL Barr John R JR
Journal of proteomics 20160923
Apolipoproteins measured in plasma or serum are potential biomarkers for assessing metabolic irregularities that are associated with the development of cardiovascular disease (CVD). LC-MS/MS allows quantitative measurement of multiple apolipoproteins in the same sample run. However, the accuracy and precision of the LC-MS/MS measurement depends on the reproducibility of the enzymatic protein digestion step. With the application of an immobilized enzyme reactor (IMER), the reproducibility of the ...[more]