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Liquid biopsy using cfDNA to predict radiation therapy response in solid tumors.


ABSTRACT:

Purpose

This study explored the potential feasibility of cell-free DNA (cfDNA) in monitoring treatment response through the measurement of chromosomal instabilities using I-scores in the context of radiation therapy (RT) for other solid tumors.

Materials and methods

This study enrolled 23 patients treated with RT for lung, esophageal, and head and neck cancer. Serial cfDNA monitoring was performed before RT, 1 week after RT, and 1 month after RT. Low-depth whole-genome sequencing was done using Nano kit and NextSeq 500 (Illumina Inc.). To measure the extent of genome-wide copy number instability, I-score was calculated.

Results

Pretreatment I-score was elevated to more than 5.09 in 17 patients (73.9%). There was a significant positive correlation between the gross tumor volume and the baseline I-score (Spearman rho = 0.419, p = 0.047). The median I-scores at baseline, post-RT 1 week (P1W), and post-RT 1 month (P1M) were 5.27, 5.13, and 4.79, respectively. The I-score at P1M was significantly lower than that at baseline (p = 0.002), while the difference between baseline and P1W was not significant (p = 0.244).

Conclusion

We have shown the feasibility of cfDNA I-score to detect minimal residual disease after RT in patients with lung cancer, esophageal cancer, and head and neck cancer. Additional studies are ongoing to optimize the measurement and analysis of I-scores to predict the radiation response in cancer patients.

SUBMITTER: Cho WK 

PROVIDER: S-EPMC10073841 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Publications

Liquid biopsy using cfDNA to predict radiation therapy response in solid tumors.

Cho Won Kyung WK   Lee Junnam J   Youn Sung-Min SM   Oh Dongryul D   Lim Do Hoon DH   Yoon Han Gyul HG   Cho Eun-Hae EH   Noh Jae Myoung JM  

Radiation oncology journal 20230328 1


<h4>Purpose</h4>This study explored the potential feasibility of cell-free DNA (cfDNA) in monitoring treatment response through the measurement of chromosomal instabilities using I-scores in the context of radiation therapy (RT) for other solid tumors.<h4>Materials and methods</h4>This study enrolled 23 patients treated with RT for lung, esophageal, and head and neck cancer. Serial cfDNA monitoring was performed before RT, 1 week after RT, and 1 month after RT. Low-depth whole-genome sequencing  ...[more]

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