Liquid biopsy using cfDNA to predict radiation therapy response in solid tumors.

ABSTRACT:

Purpose

This study explored the potential feasibility of cell-free DNA (cfDNA) in monitoring treatment response through the measurement of chromosomal instabilities using I-scores in the context of radiation therapy (RT) for other solid tumors.

Materials and methods

This study enrolled 23 patients treated with RT for lung, esophageal, and head and neck cancer. Serial cfDNA monitoring was performed before RT, 1 week after RT, and 1 month after RT. Low-depth whole-genome sequencing was done using Nano kit and NextSeq 500 (Illumina Inc.). To measure the extent of genome-wide copy number instability, I-score was calculated.

Results

Pretreatment I-score was elevated to more than 5.09 in 17 patients (73.9%). There was a significant positive correlation between the gross tum

SUBMITTER: Cho WK 

PROVIDER: S-EPMC10073841 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature