New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota.

ABSTRACT: Uncultured microbes represent a huge untapped biological resource of novel genes and gene products. Although recent genomic and metagenomic sequencing efforts have led to the identification of numerous genes that are homologous to existing annotated genes, there remains, yet, an enormous pool of unannotated genes that do not find significant sequence homology to existing annotated genes. Functional metagenomics offers a way to identify and annotate novel gene products. Here, we use functional metagenomics to mine novel carbohydrate binding domains that might aid human gut commensals in adherence, gut colonization, and metabolism of complex carbohydrates. We report the construction and functional screening of a metagenomic phage display library from healthy human fecal samples against dieta

SUBMITTER: Akhtar A 

PROVIDER: S-EPMC10076258 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature