An optimized circular polymerase extension reaction-based method for functional analysis of SARS-CoV-2.

ABSTRACT:

Background

Reverse genetics systems have been crucial for studying specific viral genes and their relevance in the virus lifecycle, and become important tools for the rational attenuation of viruses and thereby for vaccine design. Recent rapid progress has been made in the establishment of reverse genetics systems for functional analysis of SARS-CoV-2, a coronavirus that causes the ongoing COVID-19 pandemic that has resulted in detrimental public health and economic burden. Among the different reverse genetics approaches, circular polymerase extension reaction (CPER) has become one of the leading methodologies to generate recombinant SARS-CoV-2 infectious clones. Although CPER has greatly facilitated SARS-CoV-2 analysis, it still has certain intrinsic limitations that impede the ef

SUBMITTER: Liu G 

PROVIDER: S-EPMC10080526 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature