A single-cell, time-resolved profiling of Xenopus mucociliary epithelium reveals nonhierarchical model of development.

ABSTRACT: The specialized cell types of the mucociliary epithelium (MCE) lining the respiratory tract enable continuous airway clearing, with its defects leading to chronic respiratory diseases. The molecular mechanisms driving cell fate acquisition and temporal specialization during mucociliary epithelial development remain largely unknown. Here, we profile the developing Xenopus MCE from pluripotent to mature stages by single-cell transcriptomics, identifying multipotent early epithelial progenitors that execute multilineage cues before specializing into late-stage ionocytes and goblet and basal cells. Combining in silico lineage inference, in situ hybridization, and single-cell multiplexed RNA imaging, we capture the initial bifurcation into early epithelial and multiciliated progenitors a

SUBMITTER: Lee J 

PROVIDER: S-EPMC10081853 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature