SLICER: A Seamless Gene Deletion Method for Deinococcus radiodurans.

ABSTRACT: Deinococcus radiodurans' high resistance to various stressors combined with its ability to utilize sustainable carbon sources makes it an attractive bacterial chassis for synthetic biology and industrial bioproduction. However, to fully harness the capabilities of this microbe, further strain engineering and tool development are required. Methods for creating seamless genome modifications are an essential part of the microbial genetic toolkit to enable strain engineering. Here, we report the development of the SLICER method, which can be used to create seamless gene deletions in D. radiodurans. This process involves (a) integration of a seamless deletion cassette replacing a target gene, (b) introduction of the pSLICER plasmid to mediate cassette excision by I-SceI end

SUBMITTER: Brumwell SL 

PROVIDER: S-EPMC10085245 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature