Cancer-Specific Sequences in the Diagnosis and Treatment of NUT Carcinoma.

ABSTRACT:

Purpose

NUT carcinoma (NC) is a solid tumor caused by the rearrangement of NUTM1 that usually develops in midline structures, such as the thorax. No standard treatment has been established despite high lethality. Thus, we investigated whether targeting the junction region of NUTM1 fusion breakpoints could serve as a potential treatment option for NC.

Materials and methods

We designed and evaluated a series of small interfering RNAs (siRNAs) targeting the junction region of BRD4-NUTM1 fusion (B4N), the most common form of NUTM1 fusion. Droplet digital polymerase chain reaction using the blood of patients was also tested to evaluate the treatment responses by the junction sequence of the B4N fusion transcripts.

Results

As expected, the majority of NC fusion types were B

SUBMITTER: Lee MS 

PROVIDER: S-EPMC10101799 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature