Unknown

Dataset Information

0

A method for stabilising the XX karyotype in female mESC cultures.


ABSTRACT: Female mouse embryonic stem cells (mESCs) present differently from male mESCs in several fundamental ways; however, complications with their in vitro culture have resulted in an under-representation of female mESCs in the literature. Recent studies show that the second X chromosome in female, and more specifically the transcriptional activity from both of these chromosomes due to absent X chromosome inactivation, sets female and male mESCs apart. To avoid this undesirable state, female mESCs in culture preferentially adopt an XO karyotype, with this adaption leading to loss of their unique properties in favour of a state that is near indistinguishable from male mESCs. If female pluripotency is to be studied effectively in this system, it is crucial that high-quality cultures of XX mESCs are available. Here, we report a method for better maintaining XX female mESCs in culture that also stabilises the male karyotype and makes study of female-specific pluripotency more feasible.

SUBMITTER: Keniry A 

PROVIDER: S-EPMC10112917 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

A method for stabilising the XX karyotype in female mESC cultures.

Keniry Andrew A   Jansz Natasha N   Hickey Peter F PF   Breslin Kelsey A KA   Iminitoff Megan M   Beck Tamara T   Gouil Quentin Q   Ritchie Matthew E ME   Blewitt Marnie E ME  

Development (Cambridge, England) 20221128 22


Female mouse embryonic stem cells (mESCs) present differently from male mESCs in several fundamental ways; however, complications with their in vitro culture have resulted in an under-representation of female mESCs in the literature. Recent studies show that the second X chromosome in female, and more specifically the transcriptional activity from both of these chromosomes due to absent X chromosome inactivation, sets female and male mESCs apart. To avoid this undesirable state, female mESCs in  ...[more]

Similar Datasets

2022-10-31 | GSE162965 | GEO
| PRJNA683940 | ENA
| S-EPMC5547087 | biostudies-other
| S-EPMC6351820 | biostudies-literature
| S-EPMC5522374 | biostudies-other
| S-EPMC10453803 | biostudies-literature
| S-EPMC554793 | biostudies-literature
| S-EPMC4264747 | biostudies-literature
| S-EPMC4780077 | biostudies-literature
| PRJNA1000271 | ENA