MORC proteins regulate transcription factor binding by mediating chromatin compaction in active chromatin regions.

ABSTRACT:

Background

The microrchidia (MORC) proteins are a family of evolutionarily conserved GHKL-type ATPases involved in chromatin compaction and gene silencing. Arabidopsis MORC proteins act in the RNA-directed DNA methylation (RdDM) pathway, where they act as molecular tethers to ensure the efficient establishment of RdDM and de novo gene silencing. However, MORC proteins also have RdDM-independent functions although their underlying mechanisms are unknown.

Results

In this study, we examine MORC binding regions where RdDM does not occur in order to shed light on the RdDM-independent functions of MORC proteins. We find that MORC proteins compact chromatin and reduce DNA accessibility to transcription factors, thereby repressing gene expression. We also find that MORC-mediated rep

SUBMITTER: Zhong Z 

PROVIDER: S-EPMC10131428 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature