Methanobrevibacter boviskoreani JH1^T growth on alcohols allows development of a high throughput bioassay to detect methanogen inhibition.

ABSTRACT: Rumen methanogenic archaea use by-products of fermentation to carry out methanogenesis for energy generation. A key fermentation by-product is hydrogen (H₂), which acts as the source of reducing potential for methane (CH₄) formation in hydrogenotrophic methanogens. The in vitro cultivation of hydrogenotrophic rumen methanogens requires pressurised H₂ which limits the ability to conduct high-throughput screening experiments with these organisms. The genome of the hydrogenotrophic methanogen Methanobrevibacter boviskoreani JH1^T harbors genes encoding an NADP-dependent alcohol dehydrogenase and a F₄₂₀-dependent NADP reductase, which may facilitate the transfer of reducing potential from ethanol to F₄₂₀ via NADP. T

SUBMITTER: Li Y 

PROVIDER: S-EPMC10139955 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature