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In Vivo Neuropharmacological Effects of Neophytadiene.

ABSTRACT: Neophytadiene (NPT) is a diterpene found in the methanolic extracts of Crataeva nurvala and Blumea lacera, plants reported with anxiolytic-like activity, sedative properties, and antidepressant-like actions; however, the contribution of neophytadiene to these effects is unknown. This study determined the neuropharmacological (anxiolytic-like, antidepressant-like, anticonvulsant, and sedative) effects of neophytadiene (0.1-10 mg/kg p.o.) and determined the mechanisms of action involved in the neuropharmacological actions using inhibitors such as flumazenil and analyzing the possible interaction of neophytadiene with GABA receptors using a molecular docking study. The behavioral tests were evaluated using the light-dark box, elevated plus-maze, open field, hole-board, convulsion, tail suspension, pentobarbital-induced sleeping, and rotarod. The results showed that neophytadiene exhibited anxiolytic-like activity only to the high dose (10 mg/kg) in the elevated plus-maze and hole-board tests, and anticonvulsant actions in the 4-aminopyridine and pentylenetetrazole-induced seizures test. The anxiolytic-like and anticonvulsant effects of neophytadiene were abolished with the pre-treatment with 2 mg/kg flumazenil. In addition, neophytadiene showed low antidepressant effects (about 3-fold lower) compared to fluoxetine. On other hand, neophytadiene had no sedative or locomotor effects. In conclusion, neophytadiene exerts anxiolytic-like and anticonvulsant activities with the probable participation of the GABAergic system.

SUBMITTER: Gonzalez-Rivera ML

PROVIDER: S-EPMC10142729 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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In Vivo Neuropharmacological Effects of Neophytadiene.

Gonzalez-Rivera Maria L ML Barragan-Galvez Juan Carlos JC Gasca-Martínez Deisy D Hidalgo-Figueroa Sergio S Isiordia-Espinoza Mario M Alonso-Castro Angel Josabad AJ

Molecules (Basel, Switzerland) 20230414 8

Neophytadiene (NPT) is a diterpene found in the methanolic extracts of <i>Crataeva nurvala</i> and <i>Blumea lacera</i>, plants reported with anxiolytic-like activity, sedative properties, and antidepressant-like actions; however, the contribution of neophytadiene to these effects is unknown. This study determined the neuropharmacological (anxiolytic-like, antidepressant-like, anticonvulsant, and sedative) effects of neophytadiene (0.1-10 mg/kg p.o.) and determined the mechanisms of action invol ...[more]

PMID: 37110691

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In Vivo Neuropharmacological Effects of Neophytadiene.

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In Vivo Neuropharmacological Effects of Neophytadiene.

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