A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants.

ABSTRACT:

Background

PacBio HiFi sequencing provides highly accurate long-read sequencing datasets which are of great advantage for whole genome sequencing projects. One limitation of the method is the requirement for high quality, high molecular weight input DNA. This can be particularly challenging for plants that frequently contain common and species-specific secondary metabolites, which often interfere with downstream processes. Cape Primroses (genus Streptocarpus), are some of these recalcitrant plants and are selected here as material to develop a high quality, high molecular weight DNA extraction protocol for long read genome sequencing.

Results

We developed a DNA extraction method for PacBio HiFi sequencing for Streptocarpus grandis and Streptocarpus kentaniensis. A CTAB lysis

SUBMITTER: Nishii K 

PROVIDER: S-EPMC10148486 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature