Dataset Information

P3/P3N-PIPO of PVY interacting with BI-1 inhibits the degradation of NIb by ATG6 to facilitate virus replication in N. benthamiana.

ABSTRACT:

Introduction

Autophagy not only plays an antiviral role but also can be utilized by viruses to facilitate virus infection. However, the underlying mechanism of potato virus Y (PVY) infection against plant autophagy remains unclear. BI-1, localizing to the endoplasmic reticulum (ER), is a multifunctional protein and may affect the virus infection.

Methods

In this study, Y2H, BiFC, qRT-PCR, RNA-Seq, WB and so on were used for research.

Results

P3 and P3N-PIPO of PVY can interact with the Bax inhibitor 1 (BI-1) of N. benthamiana. However, BI-1 knockout mutant showed better growth and development ability. In addition, when the BI-1 gene was knocked out or knocked down in N. benthamiana, the PVY-infected mutant showed milder symptoms and lower virus accumulation. Analysis of transcriptome data showed that the deletion of NbBI-1 weakened the gene expression regulation induced by PVY infection and NbBI-1 may reduce the mRNA level of NbATG6 by regulated IRE1-dependent decay (RIDD) in PVY-infected N. benthamiana. The expression level of the ATG6 gene of PVY-infected WT was significantly down-regulated, relative to the PVY-infected mutant. Further results showed that ATG6 of N. benthamiana can degrade NIb, the RNA-dependent RNA polymerase (RdRp) of PVY. NbATG6 has a higher mRNA level in PVY-infected BI-1 knockout mutants than in PVY-infected WT.

Conclussion

The interaction of P3 and/or P3N-PIPO of PVY with BI-1 decrease the expression of the ATG6 gene might be mediated by RIDD, which inhibits the degradation of viral NIb and enhances viral replication.

SUBMITTER: Qing Z

PROVIDER: S-EPMC10149851 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

P3/P3N-PIPO of PVY interacting with BI-1 inhibits the degradation of NIb by ATG6 to facilitate virus replication in <i>N. benthamiana</i>.

Qing Zhen Z Ahmad Shakeel S Chen Yuemeng Y Liang Qingmin Q Zhang Lijuan L Chen Baoshan B Wen Ronghui R

Frontiers in plant science 20230417

<h4>Introduction</h4>Autophagy not only plays an antiviral role but also can be utilized by viruses to facilitate virus infection. However, the underlying mechanism of potato virus Y (PVY) infection against plant autophagy remains unclear. BI-1, localizing to the endoplasmic reticulum (ER), is a multifunctional protein and may affect the virus infection.<h4>Methods</h4>In this study, Y2H, BiFC, qRT-PCR, RNA-Seq, WB and so on were used for research.<h4>Results</h4>P3 and P3N-PIPO of PVY can inter ...[more]

PMID: 37139112

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