Glia maturation factor-γ is required for initiation and maintenance of hematopoietic stem and progenitor cells.

ABSTRACT:

Background

In vertebrates, hematopoietic stem and progenitor cells (HSPCs) emerge from hemogenic endothelium in the floor of the dorsal aorta and subsequently migrate to secondary niches where they expand and differentiate into committed lineages. Glia maturation factor γ (gmfg) is a key regulator of actin dynamics that was shown to be highly expressed in hematopoietic tissue. Our goal is to investigate the role and mechanism of gmfg in embryonic HSPC development.

Methods

In-depth bioinformatics analysis of our published RNA-seq data identified gmfg as a cogent candidate gene implicated in HSPC development. Loss and gain-of-function strategies were applied to study the biological function of gmfg. Whole-mount in situ hybridization, confocal microscopy, flow cytometry, and we

SUBMITTER: Li H 

PROVIDER: S-EPMC10150485 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature