In situ microwave fixation provides an instantaneous snapshot of the brain metabolome.

ABSTRACT: Brain glucose metabolism is highly heterogeneous among brain regions and continues postmortem. In particular, we demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection and preservation by liquid nitrogen. In contrast, we show that these postmortem changes are not observed with simultaneous animal sacrifice and in situ fixation with focused, high-power microwave. We further employ microwave fixation to define brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple brain regions, evidenced by reduced ¹³C enrichment into glycogen, glycolysis, and the tricarboxylic aci

SUBMITTER: Juras JA 

PROVIDER: S-EPMC10163000 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature