20S proteasome hydrolysis of LLVY substrates to determine preferences for moieties in its primed substrate channel.

ABSTRACT: The proteasome is an essential multi-catalytic enzyme in cells that is responsible for degrading proteins with a ubiquitin-dependent or -independent mechanism. Many activity-based probes, inhibitors, and stimulators have been developed to study or modulate the activity of the proteasome. The development of these proteasome probes or inhibitors have been based on their interaction with the amino acids of the β5 substrate channel proceeding the catalytically active threonine residue. There is potential for positive interactions with a substrate to increase selectivity or cleavage rate with the β5 substrate channel after the catalytic threonine as evidenced by the proteasome inhibitor belactosin. To study what moieties the proteasome could accept in its primed substrate channel, we developed

SUBMITTER: Muli CS 

PROVIDER: S-EPMC10165662 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature