Zinc homeostasis governed by Golgi-resident ZnT family members regulates ERp44-mediated proteostasis at the ER-Golgi interface.

ABSTRACT: Many secretory enzymes acquire essential zinc ions (Zn²⁺) in the Golgi complex. ERp44, a chaperone operating in the early secretory pathway, also binds Zn²⁺ to regulate its client binding and release for the control of protein traffic and homeostasis. Notably, three membrane transporter complexes, ZnT4, ZnT5/ZnT6 and ZnT7, import Zn²⁺ into the Golgi lumen in exchange with protons. To identify their specific roles, we here perform quantitative Zn²⁺ imaging using super-resolution microscopy and Zn²⁺-probes targeted in specific Golgi subregions. Systematic ZnT-knockdowns reveal that ZnT4, ZnT5/ZnT6 and ZnT7 regulate labile Zn²⁺ concentration at the distal, medial, and proximal Golgi, respectively, consistent with their localiza

SUBMITTER: Amagai Y 

PROVIDER: S-EPMC10170084 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature