Paired bone marrow and peripheral blood samples demonstrate lack of widespread dissemination of some CH clones.

ABSTRACT: Clonal hematopoiesis (CH) represents clonal expansion of mutated hematopoietic stem cells detectable in the peripheral blood or bone marrow through next generation sequencing. The current prevailing model posits that CH mutations detected in the peripheral blood mirror bone marrow mutations with clones widely disseminated across hematopoietic compartments. We sought to test the hypothesis that all clones are disseminated throughout hematopoietic tissues by comparing CH in hip vs peripheral blood specimens collected at the time of hip replacement surgery. Here, we show that patients with osteoarthritis have a high prevalence of CH, which involve genes encoding epigenetic modifiers and DNA damage repair pathway proteins. Importantly, we illustrate that CH, including clones with variant allel

SUBMITTER: Osman AEG 

PROVIDER: S-EPMC10172868 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature