Nanoelectrochemistry reveals how soluble Aβ₄₂ oligomers alter vesicular storage and release of glutamate.

ABSTRACT: Glutamate (Glu) is the major excitatory transmitter in the nervous system. Impairment of its vesicular release by β-amyloid (Aβ) oligomers is thought to participate in pathological processes leading to Alzheimer's disease. However, it remains unclear whether soluble Aβ₄₂ oligomers affect intravesicular amounts of Glu or their release in the brain, or both. Measurements made in this work on single Glu varicosities with an amperometric nanowire Glu biosensor revealed that soluble Aβ₄₂ oligomers first caused a dramatic increase in vesicular Glu storage and stimulation-induced release, accompanied by a high level of parallel spontaneous exocytosis, ultimately resulting in the depletion of intravesicular Glu content and greatly reduced release. Molecular biology tools and

SUBMITTER: Yang XK 

PROVIDER: S-EPMC10175745 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature