Biomarkers of Response to Low-Dose Aspirin in Familial Adenomatous Polyposis Patients.

ABSTRACT:

Background

The results of Aspirin prevention of colorectal adenomas in patients with familial adenomatous polyposis (FAP) are controversial.

Methods

We conducted a biomarker-based clinical study in eight FAP patients treated with enteric-coated low-dose Aspirin (100 mg daily for three months) to explore whether the drug targets mainly platelet cyclooxygenase (COX)-1 or affects extraplatelet cellular sources expressing COX-isozymes and/or off-target effects in colorectal adenomas.

Results

In FAP patients, low-dose Aspirin-acetylated platelet COX-1 at Serine529 (>70%) was associated with an almost complete inhibition of platelet thromboxane (TX) B₂ generation ex vivo (serum TXB₂). However, enhanced residual urinary 11-dehydro-TXB₂ and urinary PGEM, primary metabolites of TXA₂ and prostaglandin (PG)E₂, respectively, were detected in association with incomplete acetylation of COX-1 in normal colorectal biopsies and adenomas. Proteomics of adenomas showed that Aspirin significantly modulated only eight proteins. The upregulation of vimentin and downregulation of HBB (hemoglobin subunit beta) distinguished two groups with high vs. low residual 11-dehydro-TXB₂ levels, possibly identifying the nonresponders and responders to Aspirin.

Conclusions

Although low-dose Aspirin appropriately inhibited the platelet, persistently high systemic TXA₂ and PGE₂ biosynthesis were found, plausibly for a marginal inhibitory effect on prostanoid biosynthesis in the colorectum. Novel chemotherapeutic strategies in FAP can involve blocking the effects of TXA₂ and PGE₂ signaling with receptor antagonists.

SUBMITTER: Lanas A 

PROVIDER: S-EPMC10177499 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature