Residual Humidity in Paraffin-Embedded Tissue Reduces Nucleic Acid Stability.

ABSTRACT: Molecular diagnostics in healthcare relies increasingly on genomic and transcriptomic methodologies and requires appropriate tissue specimens from which nucleic acids (NA) of sufficiently high quality can be obtained. Besides the duration of ischemia and fixation type, NA quality depends on a variety of other pre-analytical parameters, such as storage conditions and duration. It has been discussed that the improper dehydration of tissue during processing influences the quality of NAs and the shelf life of fixed tissue. Here, we report on establishing a method for determining the amount of residual water in fixed, paraffin-embedded tissue (fixed by neutral buffered formalin or a non-crosslinking fixative) and its correlation to the performance of NAs in quantitative real-time polymerase cha

SUBMITTER: Abuja PM 

PROVIDER: S-EPMC10178321 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature