False positivity in break apart fluorescence in-situ hybridization due to polyploidy.

ABSTRACT:

Background

In-situ hybridization (ISH) is a diagnostic tool in the detection of chromosomal anomalies, which has important implications for diagnosis, classification and prediction of cancer therapy in various diseases. Certain thresholds of number of cells showing an aberrant pattern are commonly used to declare a sample as positive for genomic rearrangements. The phenomenon of polyploidy can be misleading in the interpretation of break apart fluorescence in-situ hybridization (FISH). The aim of this study is to investigate the impact of cell size and ploidy on FISH results.

Methods

In sections of varying thickness of control liver tissue and non-small cell lung cancer cases, nuclear size was measured and the number of MET chromogenic ISH and ALK FISH (liver)

SUBMITTER: van Gulik AL 

PROVIDER: S-EPMC10183404 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature