The importance of m⁶A topology in chicken embryo mRNA: a precise mapping of m⁶A at the conserved chicken β-actin zipcode.

ABSTRACT: N6-methyladenosine (m⁶A) in mRNA regulates almost every stage in the mRNA life cycle, and the development of methodologies for the high-throughput detection of methylated sites in mRNA using m⁶A-specific methylated RNA immunoprecipitation with next-generation sequencing (MeRIPSeq) or m⁶A individual-nucleotide-resolution cross-linking and immunoprecipitation (miCLIP) have revolutionized the m⁶A research field. Both of these methods are based on immunoprecipitation of fragmented mRNA. However, it is well documented that antibodies often have nonspecific activities, thus verification of identified m⁶A sites using an antibody-independent method would be highly desirable. We mapped and quantified the m⁶A site in the chicke

SUBMITTER: Baron F 

PROVIDER: S-EPMC10187669 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature