Gβγ activates PIP2 hydrolysis by recruiting and orienting PLCβ on the membrane surface.

ABSTRACT: Phospholipase C-βs (PLCβs) catalyze the hydrolysis of phosphatidylinositol 4, 5-bisphosphate [Formula: see text] into [Formula: see text] [Formula: see text] and [Formula: see text]  [Formula: see text]. [Formula: see text] regulates the activity of many membrane proteins, while IP3 and DAG lead to increased intracellular Ca²⁺ levels and activate protein kinase C, respectively. PLCβs are regulated by G protein-coupled receptors through direct interaction with [Formula: see text] and [Formula: see text] and are aqueous-soluble enzymes that must bind to the cell membrane to act on their lipid substrate. This study addresses the mechanism by which [Formula: see text] activates PLCβ3. We show that PLCβ3 functions as a slow Michaelis-Menten e

SUBMITTER: Falzone ME 

PROVIDER: S-EPMC10194004 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature