Ontology highlight
ABSTRACT:
SUBMITTER: Zhuang Q
PROVIDER: S-EPMC10195145 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
Zhuang Qian Q Ge Meiqiong M Yu Xiaodong X Chai Jing J Zhang Lu L Tang Suming S Wei Hua H Zhang Jinxin J Zhao Guojie G
Acta biochimica et biophysica Sinica 20230401 4
Rolling circle amplification is a widely used biosensing technique. Although various secondary structures have been employed in RCA, their effects on RCA efficiency have seldom been reported. Here, we find that stems in circular templates can strongly inhibit RCA, and the primer-stem distance is responsible for the inhibition. Based on the results, we propose an initiation inhibition mechanism and present a design principle for a general RCA assay. Inspired by this mechanism, we further propose ...[more]