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Higher In Vivo Fecal Concentrations of Clostridioides difficile Toxins A and B in Patients With North American Pulsed-Field Gel Electrophoresis Type 1/Ribotype 027 Strain Infection.

ABSTRACT: Ultrasensitive, quantitative Clostridioides difficile stool toxin measurement demonstrated significantly higher concentrations of toxins A and B in patients infected with the North American pulsed-field gel electrophoresis type 1/ribotype 027 (NAP-1/027) strain compared with other strains, providing in vivo confirmation of the in vitro association between NAP-1/027 and elevated toxin production.

SUBMITTER: Alonso CD

PROVIDER: S-EPMC10200326 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Higher In Vivo Fecal Concentrations of Clostridioides difficile Toxins A and B in Patients With North American Pulsed-Field Gel Electrophoresis Type 1/Ribotype 027 Strain Infection.

Alonso Carolyn D CD Pollock Nira R NR Garey Kevin W KW Gonzales-Luna Anne J AJ Williams David N DN Daugherty Kaitlyn K Cuddemi Christine C Villafuerte-Gálvez Javier J White Nicole C NC Chen Xinhua X Xu Hua H Sprague Rebecca R Barrett Caitlin C Miller Mark M Foussadier Agnès A Lantz Aude A Banz Alice A Kelly Ciarán P CP

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 20221101 11

Ultrasensitive, quantitative Clostridioides difficile stool toxin measurement demonstrated significantly higher concentrations of toxins A and B in patients infected with the North American pulsed-field gel electrophoresis type 1/ribotype 027 (NAP-1/027) strain compared with other strains, providing in vivo confirmation of the in vitro association between NAP-1/027 and elevated toxin production. ...[more]

PMID: 35607815

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Impact of CodY protein on metabolism, sporulation and virulence in Clostridioides difficile ribotype 027.

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Epidemic <i>Clostridioides difficile</i> Ribotype 027 Lineages: Comparisons of Texas Versus Worldwide Strains.

Project description:BackgroundThe epidemic Clostridioides difficile ribotype 027 strain resulted from the dissemination of 2 separate fluoroquinolone-resistant lineages: FQR1 and FQR2. Both lineages were reported to originate in North America; however, confirmatory large-scale investigations of C difficile ribotype 027 epidemiology using whole genome sequencing has not been undertaken in the United States.MethodsWhole genome sequencing and single-nucleotide polymorphism (SNP) analysis was performed on 76 clinical ribotype 027 isolates obtained from hospitalized patients in Texas with C difficile infection and compared with 32 previously sequenced worldwide strains. Maximum-likelihood phylogeny based on a set of core genome SNPs was used to construct phylogenetic trees investigating strain macro- and microevolution. Bayesian phylogenetic and phylogeographic analyses were used to incorporate temporal and geographic variables with the SNP strain analysis.ResultsWhole genome sequence analysis identified 2841 SNPs including 900 nonsynonymous mutations, 1404 synonymous substitutions, and 537 intergenic changes. Phylogenetic analysis separated the strains into 2 prominent groups, which grossly differed by 28 SNPs: the FQR1 and FQR2 lineages. Five isolates were identified as pre-epidemic strains. Phylogeny demonstrated unique clustering and resistance genes in Texas strains indicating that spatiotemporal bias has defined the microevolution of ribotype 027 genetics.ConclusionsClostridioides difficile ribotype 027 lineages emerged earlier than previously reported, coinciding with increased use of fluoroquinolones. Both FQR1 and FQR2 ribotype 027 epidemic lineages are present in Texas, but they have evolved geographically to represent region-specific public health threats.

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The largely unnoticed spread of <i>Clostridioides difficile</i> PCR ribotype 027 in Germany after 2010.

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Comparison of two capillary gel electrophoresis systems for Clostridium difficile ribotyping, using a panel of ribotype 027 isolates and whole-genome sequences as a reference standard.

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Surface layer protein A variant of Clostridium difficile PCR-ribotype 027.

Project description: Not available

| S-EPMC3204749 | biostudies-literature

Environmental transmission of Clostridioides difficile ribotype 027 at a long-term care facility; an outbreak investigation guided by whole genome sequencing.

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WGS of Clostridioides (Clostridium) difficile ribotype 027

Project description:Molecular characterization of an outbreak of Clostridioides (Clostridium) difficile ribotype 027

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Clostridioides difficile toxins deregulate tight junction proteins.

Project description:The Clostridioides difficile toxins TcdA and TcdB are responsible for diarrhea and colitis. The aim of this project was to explore the effects of the toxins on epithelial barrier function and the molecular mechanisms for diarrhea and inflammation. RNA-seq of toxin-treated intestinal cell monolayers was performed to describe the C. difficile-mediated effects. mRNA profiles from intestinale epithelial cells were generated by deep sequencing using Illumina NovaSeq 6000. This data provide the basis for subsequent upstream regulator analysis.

2023-11-29 | GSE232704 | GEO

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