Spatially resolved multimarker evaluation of CD274 (PD-L1)/PDCD1 (PD-1) immune checkpoint expression and macrophage polarisation in colorectal cancer.

ABSTRACT:

Background

The CD274 (PD-L1)/PDCD1 (PD-1) immune checkpoint interaction may promote cancer progression, but the expression patterns and prognostic significance of PD-L1 and PD-1 in the colorectal cancer microenvironment are inadequately characterised.

Methods

We used a custom 9-plex immunohistochemistry assay to quantify the expression patterns of PD-L1 and PD-1 in macrophages, T cells, and tumour cells in 910 colorectal cancer patients. We evaluated cancer-specific mortality according to immune cell subset densities using multivariable Cox regression models.

Results

Compared to PD-L1^- macrophages, PD-L1⁺ macrophages were more likely M1-polarised than M2-polarised and located closer to tumour cells. PD-L1⁺ macrophage density in the inv

SUBMITTER: Elomaa H 

PROVIDER: S-EPMC10205752 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature