Unknown

Dataset Information

0

Alpha-lipoic acid attenuates heat stress-induced apoptosis via upregulating the heat shock response in porcine parthenotes.


ABSTRACT: Heat stress (HS) is a long-standing hurdle that animals face in the living environment. Alpha-lipoic acid (ALA) is a strong antioxidant synthesized by plants and animals. The present study evaluated the mechanism of ALA action in HS-induced early porcine parthenotes development. Parthenogenetically activated porcine oocytes were divided into three groups: control, high temperature (HT) (42 °C for 10 h), and HT + ALA (with 10 µM ALA). The results show that HT treatment significantly reduced the blastocyst formation rate compared to the control. The addition of ALA partially restored the development and improved the quality of blastocysts. Moreover, supplementation with ALA not only induced lower levels of reactive oxygen species and higher glutathione levels but also markedly reduced the expression of glucose regulatory protein 78. The protein levels of heat shock factor 1 and heat shock protein 40 were higher in the HT + ALA group, which suggests activation of the heat shock response. The addition of ALA reduced the expression of caspase 3 and increased the expression of B-cell lymphoma-extra-large protein. Collectively, this study revealed that ALA supplementation ameliorated HS-induced apoptosis by suppressing oxidative and endoplasmic reticulum stresses via activating the heat shock response, which improved the quality of HS-exposed porcine parthenotes.

SUBMITTER: Lee SH 

PROVIDER: S-EPMC10209172 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Alpha-lipoic acid attenuates heat stress-induced apoptosis via upregulating the heat shock response in porcine parthenotes.

Lee Song-Hee SH   Sun Ming-Hong MH   Jiang Wen-Jie WJ   Li Xiao-Han XH   Heo Geun G   Zhou Dongjie D   Chen Zhi Z   Cui Xiang-Shun XS  

Scientific reports 20230524 1


Heat stress (HS) is a long-standing hurdle that animals face in the living environment. Alpha-lipoic acid (ALA) is a strong antioxidant synthesized by plants and animals. The present study evaluated the mechanism of ALA action in HS-induced early porcine parthenotes development. Parthenogenetically activated porcine oocytes were divided into three groups: control, high temperature (HT) (42 °C for 10 h), and HT + ALA (with 10 µM ALA). The results show that HT treatment significantly reduced the b  ...[more]

Similar Datasets

| S-EPMC7687893 | biostudies-literature
2024-05-23 | PXD045233 | Pride
| S-EPMC7189383 | biostudies-literature
| S-EPMC8312949 | biostudies-literature
| S-EPMC1805485 | biostudies-literature
| S-EPMC7298987 | biostudies-literature
| S-EPMC4547983 | biostudies-literature
| S-EPMC6541350 | biostudies-literature
| S-EPMC5960005 | biostudies-literature
| S-EPMC4532939 | biostudies-literature