DNA-Origami Line-Actants Control Domain Organization and Fission in Synthetic Membranes.

ABSTRACT: Cells can precisely program the shape and lateral organization of their membranes using protein machinery. Aiming to replicate a comparable degree of control, here we introduce DNA-origami line-actants (DOLAs) as synthetic analogues of membrane-sculpting proteins. DOLAs are designed to selectively accumulate at the line-interface between coexisting domains in phase-separated lipid membranes, modulating the tendency of the domains to coalesce. With experiments and coarse-grained simulations, we demonstrate that DOLAs can reversibly stabilize two-dimensional analogues of Pickering emulsions on synthetic giant liposomes, enabling dynamic programming of membrane lateral organization. The control afforded over membrane structure by DOLAs extends to three-dimensional morphology, as exemplified b

SUBMITTER: Rubio-Sanchez R 

PROVIDER: S-EPMC10214452 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature