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Functional Characterization of the Putative POT from Clostridium perfringens.


ABSTRACT: Proton-coupled oligopeptide transporters (POTs) are a fundamental part of the cellular transport machinery that provides plants, bacteria, and mammals with nutrition in the form of short peptides. However, POTs are not restricted to peptide transport; mammalian POTs have especially been in focus due to their ability to transport several peptidomimetics in the small intestine. Herein, we studied a POT from Clostridium perfringens (CPEPOT), which unexpectedly exhibited atypical characteristics. First, very little uptake of a fluorescently labelled peptide β-Ala-Lys-AMCA, an otherwise good substrate of several other bacterial POTs, was observed. Secondly, in the presence of a competitor peptide, enhanced uptake of β-Ala-Lys-AMCA was observed due to trans-stimulation. This effect was also observed even in the absence of a proton electrochemical gradient, suggesting that β-Ala-Lys-AMCA uptake mediated by CPEPOT is likely through the substrate-concentration-driving exchange mechanism, unlike any other functionally characterized bacterial POTs.

SUBMITTER: Gharabli H 

PROVIDER: S-EPMC10215352 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Functional Characterization of the Putative POT from <i>Clostridium perfringens</i>.

Gharabli Hani H   Rafiq Maria M   Iqbal Anna A   Yan Ruyu R   Aduri Nanda G NG   Sharma Neha N   Prabhala Bala K BK   Mirza Osman O  

Biology 20230426 5


Proton-coupled oligopeptide transporters (POTs) are a fundamental part of the cellular transport machinery that provides plants, bacteria, and mammals with nutrition in the form of short peptides. However, POTs are not restricted to peptide transport; mammalian POTs have especially been in focus due to their ability to transport several peptidomimetics in the small intestine. Herein, we studied a POT from <i>Clostridium perfringens</i> (CPEPOT), which unexpectedly exhibited atypical characterist  ...[more]

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