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Visualization and identification of benzylisoquinoline alkaloids in various nelumbo nucifera tissues

ABSTRACT: Benzylisoquinoline alkaloids in lotus (Nelumbo nucifera) seed plumules and leaves exhibit significant tissue specificity for their pharmacological effects and potential nutritional properties. Herein, 46 benzylisoquinoline alkaloids were identified via UPLC-QTOF-HRMS, of which 9 were annotated as glycosylated monobenzylisoquinoline alkaloids concentrated in the seed plumules. The spatial distribution of targeted benzylisoquinoline alkaloids in leaves, seed plumules, and milky sap was determined via MALDI–MSI. Furthermore, 37 Nelumbo cultivars were investigated using targeted metabolomics to provide insights into functional tea development. While aporphine alkaloids comprised the main compounds present in lotus leaves, bisbenzylisoquinoline alkaloids were the main compounds in lotus plumules, where glycosylation primarily occurs. These findings can help understand the distribution of benzylisoquinoline alkaloids in lotus tissue and the directional breeding of varieties enriched with specific chemical functional groups for nutritional and pharmacological applications. Highlights • Using UPLC-QTOF-HRMSn, 46 alkaloids were identified in lotus.• Among the 46 alkaloids, 9 were glycosylated monobenzylisoquinolines.• Their tissue distribution was investigated using MALDI-MSI.• Natural monobenzylisoquinoline alkaloid glycosylation enriched the glycosylated alkaloids in a tissue-specific manner.• Our findings provide insights into the biosynthesis of benzylisoquinoline alkaloids.

SUBMITTER: Hao C

PROVIDER: S-EPMC10220311 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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