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Ultra-Fast Impedimetric Immunoassay for Detection of Streptococcus agalactiae Using Carbon Electrode with Nanodiamonds Film.

ABSTRACT: This publication presents the results of work on the development of a quick and cheap electrochemical immunosensor for the diagnosis of infections with the pathogen Streptococcus agalactiae. The research was carried out on the basis of the modification of the well-known glassy carbon (GC) electrodes. The surface of the GC (glassy carbon) electrode was covered with a film made of nanodiamonds, which increased the number of sites for the attachment of anti-Streptococcus agalactiae antibodies. The GC surface was activated with EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide). Determination of electrode characteristics after each modification step, performed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).

SUBMITTER: Bigus D

PROVIDER: S-EPMC10222630 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Ultra-Fast Impedimetric Immunoassay for Detection of Streptococcus agalactiae Using Carbon Electrode with Nanodiamonds Film.

Bigus Daniel D Lewandowska Wioleta W Bięga Ewelina E Grela Anna A Siedlar Aleksandra A Sosnowska Marta M Fabisiak Magdalena M Łęga Tomasz T Dashkievich Yanina Y Nowacka-Dośpiał Joanna J Palka Katarzyna K Żołędowska Sabina S Nidzworski Dawid D

Micromachines 20230519 5

This publication presents the results of work on the development of a quick and cheap electrochemical immunosensor for the diagnosis of infections with the pathogen Streptococcus agalactiae. The research was carried out on the basis of the modification of the well-known glassy carbon (GC) electrodes. The surface of the GC (glassy carbon) electrode was covered with a film made of nanodiamonds, which increased the number of sites for the attachment of anti-Streptococcus agalactiae an ...[more]

PMID: 37241699

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Project description:Selenite (Se4+), a significant source of water pollution above the permissible limits, is considered a valuable metal by environmentalists. In this study, we described a novel electrochemical sensor that utilized a carbon paste electrode (CPE) that was modified using multiwall carbon nanotubes (MWCNTs) and poly(1-aminoanthraquinone) (p-AAQ) for finding Se4+ in water samples. Electrochemical quantification of Se4+ depends on the formation of a selective complex (piaselenol) with p-AAQ. In this work, we prepared a CPE modified by physical embedding of MWCNTs and 1-aminoanthraquione (AAQ), while the polymer film was formed by anodic polymerization of AAQ by applying a constant potential of 0.75 V in 0.1 M HCl for 20 s followed by cyclic voltammetry (CV) from -0.2 to 1.4 V for 20 cycles. The modified CPE was used for differential pulse voltammetry (DPV) of Se4+ in 0.1 M H2SO4 from 0 to 0.4 V with a characteristic peak at 0.27 V. Further, the proposed sensor was characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and electrochemical impedance spectroscopy (EIS). The analytical conditions regarding the electrode performance and voltammetric measurements were optimized, with the accumulation time and potential, supporting electrolyte, differential-pulse period/time, and amplitude. The EIS results indicated that the p-AAQ/MWCNTs-modified CPE sensor (p-AAQ/MWCNTs/CPE) that also exhibited low charge-transfer resistance (R ct) toward the anodic stripping of Se4+, exhibited good analytical performance toward different concentrations of Se4+ in a linear range of 5-50 μg L-1 Se4+ with a limit of determination (LOD) of 1.5 μg L-1 (3σ). Furthermore, differential-pulse voltammetry was employed to determine different concentrations of Se4+ in a linear range of 1-50 μg L-1 Se4+, and an LOD value of 0.289 μg L-1 was obtained. The proposed sensor demonstrated good precision (relative standard deviation = 4.02%) at a Se4+ concentration of 5 μg L-1. Moreover, the proposed sensor was applied to analyze Se4+ in wastewater samples that were spiked with Se, and it achieved good recovery values.

Dataset Information

Ultra-Fast Impedimetric Immunoassay for Detection of Streptococcus agalactiae Using Carbon Electrode with Nanodiamonds Film.

Publications

Ultra-Fast Impedimetric Immunoassay for Detection of <i>Streptococcus agalactiae</i> Using Carbon Electrode with Nanodiamonds Film.

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