Evaluation of an enzyme immunoassay and immunodiffusion for detection of anti-Histoplasma antibodies in serum from cats and dogs.

ABSTRACT:

Background

Histoplasma antigen and anti-Histoplasma antibody detection are used to support the diagnosis of histoplasmosis. There is a paucity of published data on antibody assays.

Objectives

Our primary hypothesis was that anti-Histoplasma immunoglobulin G (IgG) antibody detection using enzyme immunoassay (EIA) will be more sensitive as compared to immunodiffusion (ID).

Animals

Thirty-seven cats and 22 dogs with proven or probable histoplasmosis; 157 negative control animals.

Methods

Residual stored sera were tested for anti-Histoplasma antibodies using EIA and ID. Results of urine antigen EIA were reviewed retrospectively. Diagnostic sensitivity was calculated for all three assays and compared between immunoglobulin G (IgG) EIA and ID. The diagnostic sensitiv

SUBMITTER: Tims R 

PROVIDER: S-EPMC10229331 | biostudies-literature | 2023 May-Jun

REPOSITORIES: biostudies-literature