Circ_0003356 suppresses gastric cancer growth through targeting the miR-668-3p/SOCS3 axis.

ABSTRACT:

Background

Circular RNAs (circRNAs) have attracted extensive attention as therapeutic targets in gastric cancer (GC). Circ_0003356 is known to be downregulated in GC tissues, but its cellular function and mechanisms remain undefined.

Aim

To investigate the role of circ_0003356 in GC at the molecular and cellular level.

Methods

Circ_0003356, miR-668-3p, and SOCS3 expression were assessed via quantitative real time-polymerase chain reaction (qRT-PCR). Wound healing, EdU, CCK-8, flow cytometry and transwell assays were used to analyze the migration, proliferation, viability, apoptosis and invasion of GC cells. The subcellular localization of circ_0003356 was monitored using fluorescence in situ hybridization. The interaction of circ_0003356 with miR-668-3p was con

SUBMITTER: Li WD 

PROVIDER: S-EPMC10237019 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature