Dataset Information

Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens.

ABSTRACT:

Objective

This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens.

Methods

The isolated bacterium NT04 using 16S rRNA gene sequencing, was identified as Enterococcus faecium. In this study, the whole genome of Enterococcus faecium NT04 was sequenced and annotated by bioinformatics analysis tools.

Results

Numerous genes encoding the production of diverse metabolic and probiotic properties, such as bacteriocin-like inhibitory substances (Enterocin A and B), cofactors, antioxidants, and vitamins, were confirmed by genomic analysis. There were no pathogenicity islands or plasmid insertions found. This strain is virulent for host colonization rather than invasion.

Conclusion

Genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.

SUBMITTER: Tarek N

PROVIDER: S-EPMC10245172 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Genome sequencing of <i>Enterococcus faecium</i> NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens.

Tarek Nashwa N Azmy Ahmed F AF Khairalla Ahmed S AS Abdel-Fattah Medhat M Jefri Ohoud A OA Shaban Mohamed M El-Sayed Abdelaziz A A AAA El-Gendy Ahmed O AO

Heliyon 20230524 6

<h4>Objective</h4>This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens.<h4>Methods</h4>The isolated bacterium NT04 using 16S rRNA gene sequencing, was identified as <i>Enterococcus faecium</i>. In this study, the whole genome of <i>Enterococcus faecium</i> NT04 was sequenced and annotated by bioinformatics analysis tools.<h4>Results</h4>Numerous genes ...[more]

PMID: 37292348

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