CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9.
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ABSTRACT: Background
Clock circadian regulator (CLOCK) is a core factor of the mammalian biological clock system in regulating female fertility and ovarian physiology. However, CLOCK's specific function and molecular mechanism in porcine granulosa cells (GCs) remain unclear. In this study, we focused on CLOCK's effects on GC proliferation.Results
CLOCK significantly inhibited cell proliferation in porcine GCs. CLOCK decreased the expression of cell cycle-related genes, including CCNB1, CCNE1, and CDK4 at the mRNA and protein levels. CDKN1A levels were upregulated by CLOCK. ASB9 is a newly-identified target of CLOCK that inhibits GC proliferation; CLOCK binds to the E-box element in the ASB9 promoter.Conclusions
These findings suggest that CLOCK inhibits the proliferation of porcine ovarian GCs by increasing ASB9 level.
SUBMITTER: Huang L
PROVIDER: S-EPMC10245596 | biostudies-literature | 2023 Jun
REPOSITORIES: biostudies-literature
Publications
CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9.
Huang Liang L Yuan Huan H Shi Shengjie S Song Xiangrong X Zhang Lutong L Zhou Xiaoge X Gao Lei L Pang Weijun W Yang Gongshe G Chu Guiyan G
Journal of animal science and biotechnology 20230607 1
<h4>Background</h4>Clock circadian regulator (CLOCK) is a core factor of the mammalian biological clock system in regulating female fertility and ovarian physiology. However, CLOCK's specific function and molecular mechanism in porcine granulosa cells (GCs) remain unclear. In this study, we focused on CLOCK's effects on GC proliferation.<h4>Results</h4>CLOCK significantly inhibited cell proliferation in porcine GCs. CLOCK decreased the expression of cell cycle-related genes, including CCNB1, CCN ...[more]
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