Dataset Information

Cross-Protection Induced by Highly Conserved Human B, CD4^+, and CD8⁺ T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern.

ABSTRACT:

Background

The Coronavirus disease 2019 (COVID-19) pandemic has created one of the largest global health crises in almost a century. Although the current rate of SARS-CoV-2 infections has decreased significantly; the long-term outlook of COVID-19 remains a serious cause of high death worldwide; with the mortality rate still surpassing even the worst mortality rates recorded for the influenza viruses. The continuous emergence of SARS-CoV-2 variants of concern (VOCs), including multiple heavily mutated Omicron sub-variants, have prolonged the COVID-19 pandemic and outlines the urgent need for a next-generation vaccine that will protect from multiple SARS-CoV-2 VOCs.

Methods

In the present study, we designed a multi-epitope-based Coronavirus vaccine that incorporated B, CD4⁺, and CD8⁺ T cell epitopes conserved among all known SARS-CoV-2 VOCs and selectively recognized by CD8⁺ and CD4⁺ T-cells from asymptomatic COVID-19 patients irrespective of VOC infection. The safety, immunogenicity, and cross-protective immunity of this pan-Coronavirus vaccine were studied against six VOCs using an innovative triple transgenic h-ACE-2-HLA-A2/DR mouse model.

Results

The Pan-Coronavirus vaccine: (i) is safe; (ii) induces high frequencies of lung-resident functional CD8⁺ and CD4⁺ T_EM and T_RM cells; and (iii) provides robust protection against virus replication and COVID-19-related lung pathology and death caused by six SARS-CoV-2 VOCs: Alpha (B.1.1.7), Beta (B.1.351), Gamma or P1 (B.1.1.28.1), Delta (lineage B.1.617.2) and Omicron (B.1.1.529).

Conclusions

A multi-epitope pan-Coronavirus vaccine bearing conserved human B and T cell epitopes from structural and non-structural SARS-CoV-2 antigens induced cross-protective immunity that cleared the virus, and reduced COVID-19-related lung pathology and death caused by multiple SARS-CoV-2 VOCs.

SUBMITTER: Prakash S

PROVIDER: S-EPMC10245830 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature

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Publications

Cross-Protection Induced by Highly Conserved Human B, CD4<sup>+,</sup> and CD8<sup>+</sup> T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern.

Prakash Swayam S Dhanushkodi Nisha R NR Zayou Latifa L Ibraim Izabela Coimbra IC Quadiri Afshana A Coulon Pierre Gregoire PG Tifrea Delia F DF Suzler Berfin B Amin Mohamed M Chilukuri Amruth A Edwards Robert A RA Vahed Hawa H Nesburn Anthony B AB Kuppermann Baruch D BD Ulmer Jeffrey B JB Gil Daniel D Jones Trevor M TM BenMohamed Lbachir L

bioRxiv : the preprint server for biology 20230524

<h4>Background</h4>The Coronavirus disease 2019 (COVID-19) pandemic has created one of the largest global health crises in almost a century. Although the current rate of SARS-CoV-2 infections has decreased significantly; the long-term outlook of COVID-19 remains a serious cause of high death worldwide; with the mortality rate still surpassing even the worst mortality rates recorded for the influenza viruses. The continuous emergence of SARS-CoV-2 variants of concern (VOCs), including multiple he ...[more]

PMID: 37292861

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Dataset Information

Cross-Protection Induced by Highly Conserved Human B, CD4^+, and CD8⁺ T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern.

Background

Methods

Results

Conclusions

Publications

Cross-Protection Induced by Highly Conserved Human B, CD4<sup>+,</sup> and CD8<sup>+</sup> T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern.

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Tweets

Dataset Information

Cross-Protection Induced by Highly Conserved Human B, CD4+, and CD8+ T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern.

Background

Methods

Results

Conclusions

Publications

Cross-Protection Induced by Highly Conserved Human B, CD4<sup>+,</sup> and CD8<sup>+</sup> T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Cross-Protection Induced by Highly Conserved Human B, CD4^+, and CD8⁺ T Cell Epitopes-Based Coronavirus Vaccine Against Severe Infection, Disease, and Death Caused by Multiple SARS-CoV-2 Variants of Concern.