Serratia marcescens in the intestine of housefly larvae inhibits host growth by interfering with gut microbiota.

ABSTRACT:

Background

The structure of gut microbiota is highly complex. Insects have ubiquitous associations with intestinal symbiotic bacteria, which play essential roles. Thus, understanding how changes in the abundance of a single bacterium interfere with bacterial interactions in the insect's gut is important.

Methods

Here, we analyzed the effects of Serratia marcescens on the growth and development of housefly larvae using phage technology. We used 16S rRNA gene sequencing technology to explore dynamic diversity and variation in gut bacterial communities and performed plate confrontation assays to study the interaction between S. marcescens and intestinal microorganisms. Furthermore, we performed phenoloxidase activity assay, crawling assay, and trypan blue staining to explore th

SUBMITTER: Li Y 

PROVIDER: S-EPMC10257315 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature